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Name
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BUSINESS ADDRESS Department of Neurobiology Phone: 205-975-5573 |
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EDUCATION
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1996 |
Ph.D.,
Department of Molecular Genetics and Microbiology,
University of Florida College of Medicine, Gainesville, Florida |
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1989 |
M.S., Microbiology,
University of South Florida, Tampa, Florida |
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1986 |
B.S., Biology,
University of South Florida, Tampa, Florida |
PROFESSIONAL
EMPLOYMENT
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1997-present |
Postdoctoral Fellow, Mammalian Genetics Laboratory, NCI-Frederick Cancer Research and Development Center, Frederick, MD. Conducted research on the identification of mouse mutations which cause deafness and ataxia. Used a positional cloning approach to generate genetic and physical maps for the jerker, waltzer, ataxia, and ashen mutations. Generated transgeneic mice for the rescue of the above mutations. Conducted large-scale sequencing of the critical intervals for the ataxia and waltzer mutations. Identified the waltzer, ataxia, and ashen genes and characterized the expression of these genes using in situ hybridization and various immunological staining procedures. Constructed gene knockouts for the Cacna1e and Cacna1a voltage-gated calcium channels and initiated collaborations to study the behavior and electrophysiology of these mice. |
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1996-1997 |
Postdoctoral Fellow, Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL. Examined the function of pre-mRNA binding proteins in Saccharomyces cerevisiae. |
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1991-1996 |
Graduate Student, Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida. Studied the role of pre-mRNA binding proteins in the maturation of mRNA. Identified the first family of pre-mRNA binding proteins in Saccharomyces cerevisiae. Used a combination of cell biology, biochemistry and genetics to determine the function of pre-mRNA binding proteins in yeast. |
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1990-1991 |
Biological Scientist, Department of Immunology and Medical Microbiology, University of South Florida, Tampa, Florida. Analyzed the effects of cytokines and stress factors on Legionella pneumophila infection. Used cell culture assays and molecular biology techniques to study Legionella pneumophila infection in macrophages. |
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1990-1991 |
Instructor, Introductory Biology, Hillsboro Community College, Tampa, Florida. Developed curriculum, conducted lectures, and evaluated student performance in undergraduate Biology classes. |
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1987-1989 |
Graduate Student and Laboratory Instructor, Cell Biology and Bacteriology, University of South Florida, Tampa, Florida. Conducted research on natural transformation in gram negative bacteria. Responsible for designing and supervising laboratory classes for undergraduates in bacterial physiology and cell biology. |
RESEARCH
EXPERIENCE
MOLECULAR
BIOLOGY
Screening
genomic and cDNA expression libraries
Screening
of BAC and YAC genomic libraries
Construction
of BAC and YAC contigs
Construction
of genomic and cDNA libraries in phage and plasmid vectors
Genotyping
animals by Southern blot analysis and PCR
Northern
blot analysis and RT-PCR
Contruction
of transgenic animals
Construction
of gene knockout cassettes
Culturing
and screening embryonic stem cells
DNA
and RNA isolation from bacteria, cell culture, and animal tissues
PCR
mutagenesis for random and specific base pair changes
In
vitro transcription and translation of cloned genes
In
vivo labeling of cells with 32P, 35S and 3H.
Production
and purification of fusion proteins in E.
coli
Production
of monoclonal and polyclonal antibodies
Western
blot analysis and immunohistochemistry
GENETICS
Construction
of mitotic recombination maps in mice
Extragenic
suppressor screens
Synthetic
lethal screens
High
copy suppressor screens
Yeast
Two-Hybrid screens
Operation
and maintenance of ABI 377 automated DNA sequencer
Database
mining for identification of new genes
ANIMAL PROCEDURES
Isolation
of tissues from mice for biochemical and histological assays
Perfusions
of mice
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PUBLICATIONS Wilson,
et al. Synaptic defects in ataxia
mice result from a mutation in Usp14,
a ubiquitin-specific protease. Submitted. Wilson,
S.M., Householder, D.B., Coppola, V., Tessarollo, L., Fritzsch, B., Lee,
E.C., Goss, D., Carlson, G.A., Copeland, N.G., and Jenkins, N.A.
2001. Mutations in Cdh23
cause nonsyndromic hearing loss in waltzer
mice. Genomics
74:228-233. |
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Fletcher,
C.F., Tottene, A., Lennon, V.A., Wilson, S.M., Dubel, S.J., Paylor, R.,
Hosford, D.A., Tessarollo, L., McEnery, M.W., Pietrobon, D., Copeland, N.G.,
and Jenkins, N.A. 2001.
Dystonia and cerebellar atrophy in Cacna1a null mice lacking P/Q
calcium channel activity. FASEB J. 15:1288-1290. |
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Wilson, S.M.,
Toth, P.T., Oh, S.B., Gillard, S.E., Volsen, S., Ren, D., Philipson, L.H.,
Lee, E.C., Fletcher, C.F., Tessarollo, L., Copeland, N.G., Jenkins, N.A.,
and Miller, R.J. 2000.
The status of voltage-dependent calcium channels in µ1E
knock-out mice. J. Neurosci.
20:8566-8571. |
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Wilson, S.M.,
Yip, R., Swing, D.A., O'Sullivan, T.N., Zhang, Y., Novak, E.K., Swank, R.T.,
Russell, L.B., Copeland, N.G., and Jenkins, N.A. 2000. A mutation in Rab27a
causes the vesicle transport defects observed in ashen
mice. Proc. Natl. Acad. Sci. USA 97:7933-7938. |
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Conrad, N.K.,
Wilson, S.M., Steinmetz, E.J., Patturajan, M., Brow, D.A., Swanson, M.S.,
and Corden, J.L. 2000. A
yeast heterogeneous nuclear ribonucleoprotein complex associated with RNA
polymerase II. Genetics 154:557-571. |
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Wilson, S.M.,
Datar, K.V., Paddy, M.R., Swedlow, J.R., and Swanson, M.S.
1994. Characterization
of nuclear polyadenylated RNA-binding proteins in Saccharomyces
cerevisiae. J. Cell Biol.
127:1173-1184. |
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Anderson,
J.T., Wilson, S.M., Datar, K.V., and Swanson, M.S. 1993. NAB2:
A yeast nuclear polyadenylated RNA-binding protein essential for
cell viability. Mol. Cell.
Biol. 13: 2730-2741. |
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Klein,
T.W., Yamamoto, Y., Wilson, S., Newton, C., and Friedman, H. 1992. Legionella
pneumophila infection and cytokine production. Adv. Exp. Med. Biol.
319:97-104. |
HONORS
AND AWARDS
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2001 |
Postdoctoral Research
Competition Award, Frederick, MD |
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1996 |
First
Place, Medical Guild Research Competition Award, Gainesville, FL |
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1994 |
Graduate
Student Council Research Award, Gainesville, FL |
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